Composition
- NaCl 8.5 g
- Distilled water 1000 ml
Procedure
- Dissolve the sodium chloride in distilled water, and mix well;
- Store in a sealed container.
The quantity of salt and water can be proportionately reduced. For optimum storage:
- Distribute 10 ml aliquots in sealable glass test tubes;
- Sterilize in an autoclave at 121 °C for 15 minutes and allow to cool;
- Store at 4 °C until use.
Physiological saline should be renewed frequently and stored in sealed test tubes to prevent accidental contamination with plant spores, pollen, mould or free-living airborne amoeba. These contaminants, during microscopic examination, can be mistaken for intestinal protozoa.