(R15) Trichrome stain

Composition

-     Chromotrope 2R                                                    0.6 g

-     SF light green                                                        0.3 g

-     Phosphotungstic acid                                              0.7 g

-     Glacial acetic acid                                                 1.0 ml

-     Distilled water                                                   100.0 ml

Procedure

-     Place the solids in a 250 ml glass beaker;

-     Add acetic acid to moisten the solids and let stand for 30 minutes;

-     Add distilled water and stir carefully.

-     Store in a dark glass bottle out of sunlight.

The solution should become deep purple. It is stable for at least 1 year if stored at room temperature.  A ready to use solution is commercially available.