Composition
- Malachite green 0.2 g
- Glacial acetic acid 3.0 ml
- Ethanol 95% 3.0 ml
Procedure
- Dissolve malachite green in alcohol;
- Add acetic acid
- Add distilled water to bring the volume to 100 ml.
The solution is quite stable if stored at room temperature. For use, see: Direct microscopic examination after concentration.