REAGENTS

(R11) Sargeaunt's solution (for staining of chromatoid bodies)

Composition

-     Malachite green                                                      0.2 g

-     Glacial acetic acid                                                 3.0 ml

-     Ethanol 95%                                                        3.0 ml

Procedure

-     Dissolve malachite green in alcohol;

-     Add acetic acid

-     Add distilled water to bring the volume to 100 ml.

The solution is quite stable if stored at room temperature.  For use, see:  Direct microscopic examination after concentration.